RESUMEN
The p73 proteins are present in different kinds of cells of the central nervous system, such as the choroid plexus, circumventricular structures and neuroepithelium. It has been reported that spontaneously hypertensive rats show ventricular dilation, changes in cerebrospinal fluid proteins and variations in the circumventricular structures such as the organum vasculosum of the lamina terminalis and the choroid plexus, which are altered in ventricular dilation. The aim of the present work is to study p73 expression in the organum vasculosum of the lamina terminalis and the choroid plexus and its variations in high blood pressure. Brains from control Wistar-Kyoto rats and spontaneously hypertensive rats were used. The organum vasculosum of the lamina terminalis and the choroid plexus were processed by immunohistochemistry and western blot with anti-TAp73. We found weaker markings in the organum vasculosum of the lamina terminalis and stronger markings in the choroid plexus of the hypertensive than the control rats. Therefore, hypertension in the spontaneously hypertensive rats produces alterations in choroid plexus protein p73 expression that is similar to that described for other circumventricular organs, but it is different in the organum vasculosum of the lamina terminalis. We can conclude that the functional balance between p73, organum vasculosum of the lamina terminalis and choroid plexus, which is probably necessary to maintain the normal functioning of these structures, is altered by the hypertension found in these rats.
Asunto(s)
Plexo Coroideo/metabolismo , Proteínas de Unión al ADN/metabolismo , Regulación de la Expresión Génica , Hipotálamo/metabolismo , Proteínas Nucleares/metabolismo , Proteínas Supresoras de Tumor/metabolismo , Animales , Perfilación de la Expresión Génica , Hipertensión/metabolismo , Immunoblotting , Inmunohistoquímica , Masculino , Ratas , Ratas Endogámicas SHR , Ratas Endogámicas WKY , Proteína Tumoral p73RESUMEN
The aim of this study was to perform an immunohistochemical study of the, angiotensinergic pathway from the arcuate nucleus (AN) to the posterior lobe of the hypophysis (PLH) of 10-week-old matched normotensive Wistar Kyoto rats (WKY), using our own policlonal antibody raised in mice against Angiotensin II (mouseantiangiotensin II, MAAII). Cells and fibers in the rostroventral and dorsocaudal parts of the AN, the internal zone of the median eminence and PLH showed immunoreactive material for antiangiotensin II. Angiontensin II fibers originating in the anteroventral part of the AN, crossing median eminence (ME) and infundibular stem and arriving at the PLH were also observed (AU)
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Asunto(s)
Animales , Ratas , Núcleo Arqueado del Hipotálamo/anatomía & histología , Núcleo Arqueado del Hipotálamo/inmunología , Neurohipófisis/anatomía & histología , Neurohipófisis/inmunología , Angiotensina II/inmunología , Inmunohistoquímica/instrumentación , Inmunohistoquímica , Hipotálamo/anatomía & histologíaRESUMEN
We have studied the subcommissural organ of two hydrocephalic brains, of 20 and 21 gestational weeks and of two normal brains, aged 19 and 23 gestational weeks. Both hydrocephalic cases presented a size reduction of the subcommissural organ compared to the normal cases; only in one case, there were also alterations of the morphological components of the subcommissural organ, suggesting different pathogenic relationships between hydrocephalus and dysplasia of the subcommissural organ.
Asunto(s)
Hidrocefalia/patología , Tálamo/patología , Aborto Espontáneo/patología , Encéfalo/patología , Femenino , Humanos , Embarazo , Tálamo/embriologíaRESUMEN
Neurons in the human cerebral cortical white matter below motor, visual, auditory and prefrontal orbital areas have been studied with the Golgi method, immunohistochemistry and diaphorase histochemistry. The majority of white matter neurons are pyramidal cells displaying the typical polarized, spiny dendritic system. The morphological variety includes stellate forms as well as bipolar pyramidal cells, and the expression of a certain morphological phenotype seems to depend on the position of the neuron. Spineless nonpyramidal neurons with multipolar to bitufted dendritic fields constitute less than 10% of the neurons stained for microtubule associated protein (MAP-2). Only 3% of the MAP-2 immunoreactive neurons display nicotine adenine dinucleotide-diaphorase activity. The white matter pyramidal neurons are arranged in radial rows continuous with the columns of layer VI neurons. Neuron density is highest below layer VI, and decreases with increasing distance from the gray matter. White matter neurons are especially abundant below the primary motor cortex, and are least frequent below the visual cortex area 17. In contrast to other mammalian species, the white matter neurons in man are not only present during development, but persist throughout life.